FIGURE 6.

Effects of Sulfa4/22 on microglia activation and the release of inflammatory factors in APP/PS1 mice. (A) Detection of IBA‐1 and CD11c expression by IF staining: There was obvious activation of microglia, obvious expression of IBA‐1 and CD11c in the Con group. However, the expression of IBA‐1 and CD11c was significantly down‐regulated, and the activation of microglia was inhibited in Sulfa‐4 and Sulfa‐22 group. (B‐C) Expression of inflammatory factors in peripheral blood and CSF: The expression levels of inflammatory factors in peripheral blood and CSF were relatively high in Con group, which were significantly down‐regulated in Sulfa‐4 and Sulfa‐22 group. Comparison with Con group, *p < 0.05. (D‐I) Expression level of GSDMD and p30‐GSDMD in mouse cortex, hippocampus, and striatum: The expression GSDMD was relatively low, while p30‐GSDMD expression was relatively high in cortex, hippocampus and striatum. The cleavage of GSDMD was inhibited in Sulfa‐4 and Sulfa‐22 groups, GSDMD expression level was significantly up‐regulated, while the level of p30‐GSDMD was down‐regulated. Comparison with the Con group, *p < 0.05