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. 2021 Oct 23;25(22):10604–10613. doi: 10.1111/jcmm.16993

FIGURE 5.

FIGURE 5

CD45.1 and CD45.2 allele typing of induced lymphocytes (iLs) and fate of iLs after transfer to mice. (A) PCR analysis of genomic DNA from iLs after co‐culture of CD45.1+ HSC‐eBMCs with CD45.2+ iTECs +IL‐2 + IL‐7 for 7 days, and from residual iTECs after harvest of the iLs. Arrow indicates a minor population of CD45.2+ iPSCs or iPSC‐derived iTECs. (B) Flow cytometric analysis of CD45.1+ cells isolated from the spleen (SP), peripheral blood (PB), small intestine lymph nodes (LN), thymus (Thy) and bone marrow (BMC) of CD45.2 C57BL/6 mice after autologous BMT and injection of CD45.1+ iLs for 14 days. (C) Histology of liver, lung. kidney and small intestine (SI) sections from mice on day 31 after BMT and iLs injection. Arrows indicate portal area in the liver, bronchus in the lung, glomerulus in the kidney and crypts in the SI. (H&E staining, ×200: bar indicates 50 µm.)