FIGURE 4.

Celecoxib restored hepatic anti‐oxidative capacity via activation of the NRF2‐HO‐1 signalling pathway. (A‐C) Expression of the NRF2‐KEAP1‐HO‐1 signalling pathway was determined by immunohistochemistry (A, ×400 magnification), qRT‐PCR (B), and Western blot (C). (D‐F) Densitometry analysis of NRF2 (D), KEAP1 (E), and HO‐1 (F). G‐I: Hepatic anti‐oxidative capacity was measured as activities of enzymatic antioxidants SOD1 (G) and GSH‐Px (H), and non‐enzymatic antioxidant GSH/GSSG (I). Data are presented as mean ±SD. n=12/group, *p < 0.05 vs. Control group, ^# p < 0.05 vs. TAA group. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; GSH‐Px, glutathione peroxidase; GSSG, oxidised glutathione; HO‐1, heme oxygenase‐1; KEAP1, Kelch‐like ECH‐associated protein 1; NRF2, nuclear factor (erythroid‐derived 2)‐like 2; qRT‐PCR, quantitative real‐time PCR; SOD1, superoxide dismutase 1; TAA, thioacetamide