FIGURE 2.

Cell pyroptosis was closely associated with AP development. (A) MTT assay and (B) trypan blue staining assay were, respectively, performed to examine cell proliferation and viability in AR42J cells (each experiment was repeated for three times). (C) The expression levels of NLRP3, ASC and Gasdermin D were determined by Western Blot analysis (each experiment was repeated for three times). (D) The expression levels and localization of caspase‐1 in AR42J cells were determined by immunofluorescence staining assay. (E, F) The mRNA and (G, H) protein levels of IL‐1β and IL‐18 were measured in AR42J cells and its supernatants (each experiment was repeated for three times). *p < 0.05