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. 2021 Oct 23;25(22):10711–10723. doi: 10.1111/jcmm.17007

FIGURE 3.

FIGURE 3

PKM2 inhibition ameliorates Ang‐II‐induced late cardiac remodelling. DMSO or shikonin was given to C57BL/6J mice 3 times per week while the treatment was combined with Ang II (1,000 ng/kg/min) infusion for 14 days. (A) Diagrammatic illustration of experimental schemes and timelines. (B) Systolic blood pressures. (C) Representative M‐mode echocardiography of the left ventricle (left). Measurement of ejection fraction (EF %) and fractional shortening (FS %) (right, n = 6 per group). (D) Representative hearts (left). HW/BW and HW/TL ratios (right, n = 6 per group). (E) TRITC‐labelled wheat germ agglutinin (WGA) staining of heart sections. Scale bar, 50 μm (left). Quantification of the relative myocyte cross‐sectional area (right, n = 6 per group). (G) Masson's staining of cardiac sections. Scale bar, 50 μm (left). Quantification of the relative fibrosis (right, n = 6 per group). (F, H) qRT‐PCR analysis of mRNA expression (n = 6 per group). *p < 0.05, **p < 0.01