Figure 2.

Dpp4+ progenitor cells contribute to basal adipogenesis in subcutaneous depots. (A) Flow cytometric analysis of SVF cells (CD45(−)/DAPI(−)) from subcutaneous WAT of Dpp4^CreER; mGFP mice at the pulse timepoint. mGFP+ lineage traced cells are shown as a green overlay on top of mGFP(−) cells, which are depicted in gray. (B) IHC staining of subcutaneous inguinal WAT (iWAT) at the pulse timepoint. [mGFP: lineage traced cells (green), cells currently expressing DPP4 (magenta), PLIN1: mature adipocytes (red), DAPI: nuclei (blue); scale bar = 380 μM]. Individual antibody staining is represented in grayscale on the right-side inset panels (inset scale bar = 100 μM). (C) IHC staining of Dpp4^CreER iWAT (top panels) and axillary WAT (axWAT, bottom panels) following 18 weeks of feeding chow (left) or HFD.