Figure 3.

Dpp4+ progenitors give rise to ICAM1+/DPP4(−) preadipocytes in vivo. (A) Schematic depicting the lineage tracing timeline. Six-week-old Dpp4^CreER; mGFP mice were injected with tamoxifen to produce heritable mGFP labeling of Dpp4+ cells. Lineage traced mice were placed on either chow or HFD at 8 weeks of age and harvested at 24 weeks. (B, C) Flow cytometric analysis of mGFP+ SVF cells from visceral (B) and subcutaneous (C) WAT of Dpp4^CreER mice. CD45(−)/DAPI(−) SVF cells were gated on expression of mGFP to isolate lineage traced cells. Staining with anti-DPP4 and anti-ICAM1 at the pulse timepoint (left panel) and following 18 weeks of either chow diet (middle panel) or HFD (right panel). Data are representative of four biologic replicates. (D, E) Quantification of relative Dpp4+ lineage traced population abundance from visceral (D) and subcutaneous (E) WAT of Dpp4^CreER; mGFP mice.