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. 2021 Oct 17;11(20):9918–9936. doi: 10.7150/thno.66378

Figure 1.

Figure 1

Stimulation of melanogenesis by cAMP but not PKC signaling pathway. (A) Western blot analysis of MITF, tyrosinase, Tyrp1, and DCT in Mel-Ab cells treated with FSK or TPA for 72 h. (B) Microscopic photographs of the Mel-Ab cells treated with FSK or TPA and melanin content is shown in relative percent to basal melanin content (Bar = 20 µm). (C) Western blot analysis of p-CRTC3, CRTC3, CREB, p-CREB, and ERK in Mel-Ab cells treated with FSK or TPA for 1, 2, 6 h. αtubulin was used as a loading control, (D) Subcellular localization of CRTC3 (green color) in CRTC3-EGFP-transfected B16F10 cells treated with FSK or TPA (Bar = 200 µm), (E) The mRNA expression of NR4A2, a direct target of CREB/CRTC3, by FSK or TPA treatment within 6 h. CTRL: vehicle-treated controls, FSK: forskolin, TPA: 12-o-tetradecanoylphorbol-13-acetate