Table 1.
Representative studies for hPSC-derived BMECs
| Cell lines | Initial stage | BMEC specification | Markers | Functional assessment | Reference |
|---|---|---|---|---|---|
| IMR90-4, DF6-9-9T, DF19-9-11T, H9 |
Neural and endothelial co-differentiation | UM, hESFM, bFGF, PDS | PECAM-1, Claudin-5, GLUT-1, Pgp, VE-cadherin | TEER: ~850 Ω_cm2 LDL uptake Permeability: radiolabeled small molecules Efflux transporter activity: Pgp, MRP, BCRP Tube forming Co-culture with rat astrocytes |
67 |
| IMR90-4, DF19-9-11T, H9 | Neural and endothelial co-differentiation | UM, hESFM, bFGF, PDS, RA | GLUT-1, Claudin-5, Occludin, PECAM-1, VE-cadherin | TEER: ~5000 Ω·cm2 Permeability: radiolabeled sucrose Efflux transporter activity: Pgp, MRP, BCRP Tight junction quantification: Occludin, Claudin-5 Co-culture with human primary pericytes, neural progenitor cells, and foreskin fibroblasts |
68 |
| IMR90-4 | Neural and endothelial co-differentiation | UM, hESFM, bFGF, PDS, RA | PECAM-1 | OGD induced in vitro model of cerebral ischemia TEER Permeability Cytokine treatment |
80 |
| CS83iCTR33n1, CS14iCTR28n6, CS21iHD60n8, CS04iHD66n4, CS81iHD71n3, CS09iHD109n1 |
Neural and endothelial co-differentiation | UM, hESFM, bFGF, PDS, RA | PECAM-1, GLUT-1, Claudin-5, Occludin, ZO-1 | TEER: decreased in HD BMEC-like cells Efflux transporter activity: Pgp, ABCB1 Transcytosis: albumin Wound healing assay |
81 |
| DF19-9-11 | Neural and endothelial co-differentiation | UM, hESFM, bFGF, PDS, RA | Claudin-5, Occludin, ZO-1 | GBS infection assay TEER |
82 |
| IMR90-4, DF19-9-11T, H9 | Primitive streak-like stage | CHIR99021, bFGF, RA, B27, hESFM, | PECAM-1, ZO-1, VE-cadherin, GLUT-1, Claudin-5, Occludin, BCRP, MRP, Pgp, vWF | TEER: above 3000 Ω·cm2 LPL uptake Efflux transporter activity: Pgp, MRP, BCRP Coculture with primary pericytes, hPSC-derived neurons and astrocytes. |
73 |
| IMR90-4, CD12, SM14, CC3 | Neuroectoderm | E6, hESFM, bFGF, PDS, RA | GLUT-1, Claudin-5, Occludin, PECAM-1, VE-cadherin | TEER: above 2500 Ω·cm2 Permeability: sodium fluorescein Efflux transporter activity: Pgp, MRP Co-culture with human primary pericytes and iPSC-derived astrocytes |
70 |
| CC3, CD10, HD70-2, and TSP8-15 | Neuroectoderm | E6, hESFM, bFGF, RA, PDS, B27, ITS | PECAM-1, Claudin-5, GLUT-1, VE-cadherin, Occludin | TEER: ~3000 Ω·cm2 Permeability: sodium fluorescein Efflux transporter activity: Pgp, MRP Tight junction quantification: Occludin, Claudin-5 Co-culture with human iPSC-derived astrocytes |
71 |
| IMR90-4 | Neural and endothelial co-differentiation | Hypoxia: UM, hESFM, bFGF, PDS, RA | ZO-1, Claudin-5, PECAM-1, GLUT-1, Pgp | TEER: 25000 Ω Efflux transporter activity: Pgp, MRP1, MRP4, BCRP Permeability: 3, 10 kDa dextran |
74 |
| IMR90-4, H1, H6 | Neural and endothelial co-differentiation | UM, hESFM, bFGF, PDS, RA, transduction of FL1, ERG, and ETV2 lentiviral vectors at D6 | ZO-1, Occludin, PECAM1, CDH5, EPCAM-1 | TEER: ~200 Ω·cm2 Permeability: 70 kDa dextran Formation of a capillary network Responses to TNFα |
76 |
| CC3, CD10, CDH5-2A-eGFP | Neuroectoderm | E6, hESFM, DMEM/F12, neurobasal, bFGF, RA, B27 | GLUT-1, Claudin-5, Occludin, VE-cadherin | TEER Permeability: sodium fluorescein Efflux transporter activity: Pgp, MRP |
72 |
| IMR90-4, iPSC donor 1, 2, and 3. | CD34+CD31+ EC progenitor cells | LaSR, hESFM, B27, bFGF, RA | Claudin-5, Occludin, VE-cadherin, PECAM-1, ZO-1 | TEER Permeability: sodium fluorescein Pro-inflammatory cytokine simulation Co-culture with iPSC-derived smooth muscle-like cells, human astrocytes, hiPSC-derived astrocytes, bovine pericytes, and human brain pericytes. Tube forming in mouse |
9 |