Table 2.
Representative studies for hPSC-derived pericytes
| Cell lines | Initial stage | Pericytes specification | Markers | Functional assessment | Reference |
|---|---|---|---|---|---|
| H9.2, I6, C3, KTR13 | Spontaneously differentiation EBs | EBM-2, EC M-19, FBS | CD105, CD90, CD73, CD31, CD146, NG2, and PDGFR positive | Pericytes and EC assembly Hind limb ischemia model |
90 |
| H9, H13, clone 26 hCBiPS aMHCneoPGKhygro+, pCAGGS2, hiPSC-MR31, hESC-H9, hiPSC-BC1 | Early vascular cells 12d Pericyte inducing condition 6d |
EVC: CIV serum VEGF, SB431542 in EC media Pericytes Serum aMEM |
CD73, NG2, PDGFRβ, CD44 positive, VE-cadherin and CD31 negative | Self-organized vascular networks in collagen and HA hydrogels In vivo integration |
94, 95 |
| HESC-NL4, Fib-iPSC BOEC-iPSC, NL-HES4, HES3 (NKX2-5eGFP/w) | CD31- fraction | Activin A, BMP4, CHIR99021, VEGF, SB43152 DMEM, FBS, TGFbeta3, PDGF-BB |
PDGFRβ, CD146, NG2, CD73, CD44, CD105 | Vascular Plexus Zebrafish xenografts |
91
92 |
| ESI-017 | EB | BMP4, FGF2, Activin-A, VEGFA, SB431542 | CD146, CD73, and CD105 positive; CD31, CD34, and CD133 negative | Tube formation assay | 99 |
| H1, H9-EGFP, IISH2i-BM9 | Mesenchymal progenitors Immature pericytes |
FGF2, PDGF-BB, SB431542, VEGF, EGF | Capillary Phenotype: NG2+ α-SMAlow/-
Desminlow/-Calponin low/- MYH11-
Arteriolar phenotype: NG2high α-SMA+ Desmin+ Calponinlow/- MYH11- |
Vasculature formation in vitro and in vivo | 96 |
| BC1, C12-RFP | Mesoderm, early vascular, pericyte maturation | sB431542, VEGF, pericytes medium | PDGFRβ, NG2, CD31, Calponin | Transwell: coculture pericytes with hPSC-derived BMEC-like cells | 100 |
| AD5, AD6, AD13, AD14, AD20, AD22, AD29, H9 (WA09) and H1 (WA01) | Mesoderm Neural crest |
Mesoderm: MIM, DKK1, pericytes medium Neural crest: B27, CHIR99021, pericytes medium |
PDGFRβ, NG2, CD13, and CD146 positive | Transwell: coculture pericytes with hPSC-derived BMEC-like cells | 97 |
| H9, IMR90C4, CS03n2 | Neural crest | E6, CHIR99021, SB431542, bFGF, dorsomorphin, FBS | PDGFRβ, NG2 | Self-assembling with endothelial cells Transwell: coculture pericytes with iPSC-derived BMEC-like cells |
98 |
| H1, H9, DF19-11, 005B23.1, CD3-3, PMBC-3-1, WTC11, WT83, Q83X, M2 | Mesoderm CD34- fraction |
E8BAC medium: E8, BMP4, Activin-A, and CHIR99021 E7BVi: E8 minus TGFβ1, BMP4, VEGFA, and SB431542 |
CD34, CD31 negative, PDGFRβ, α-SMA, SM22 positive | Angiogenesis assay | 93 |