Table 3.
Representative studies for hPSC-derived astrocytes
| Cell lines | Initial stage | Astrocyte specification | Markers | Functional assessment | References |
|---|---|---|---|---|---|
| H9, H7, IMR90-4 | Neuroepithelia | RA, FGF8, SHH, CNTF, LIF, FBS | S100β, GFAP | glutamate uptake synaptogenesis Calcium wave Astrocyte-neuronal co-culture Animal transplantation |
103 |
| H9, HUES9 | bFGF, EGF | B27, BMP4, LIF | S100β, GFAP, EAAT1, aquaporin | Oxidative neuronal injury | 104 |
| WA-09, WA-01, IMR90- 4, iPS-Foreskin-1 |
N2, bFGF, FGF, EGF, CNTF, | N2, CNTF | S100β, GFAP | Migratory capacity Tropism for hHGG |
105 |
| H9, H14, BC1 | B27, bFGF, CNTF, BMP | CNTF, BMP, FBS | GFAP, TUJ1 | Integrate in vivo | 106 |
| N116213, N117322, 409B2, APP1E111, APP1E211, APP1E311, APP2E22, APP2E26, AD3E211, AD8K213 | Cortical neuron: N2, SB431542, dorsomorphin, B27, BDNF, GDNF, NT-3 |
Repeat passage to a non-coated polystyrene dish | GFAP | Accumulation of Ab oligomers | 107 |
| 4.2 line, GM003814 unaffected 21.8 line, GM002183; SMA 3.6 line, GM003813; SMA 7.12 line, GM09677 |
bFGF, EGF, | B27, CNTF | GFAP | Disease phenotypes of SMA | 108 |
| M337V-1, M337V-2, CTRL-1 iPSC line, CTRL-2 iPSC line | LIF, EGF, bFGF, | B27, CNTF | S100β, GFAP | Astrocyte-neuronal co-culture Disease phenotypes of ALS |
94 |
| H9, SeV-derived iPSC line and modRNA-derived iPSC line | N2, bFGF, EGF, FGF, CNTF, Noggin, SB431542 | CNTF | A2B5, GFAP |
In vitro migratory capacity In vivo transplantation |
109 |
| HC1, HC2, HC3, MS1, MS2, MS3, MS4, | Noggin, bFGF, SB431542 | bFGF, EGF, LIF, CNTF | S100β, GFAP, GLAST | Disease phenotypes of multiple sclerosis | 110 |
| WA09, H9, RRID:CVCL_9973,GM1-4, RRID:CVCL_7290 | N2, B27, SB431542, DMH1 | SHH, CHIR99021, CNTF, cyclopamine, prumorphamine, RA, bFGF, BMP4. | Regional markers: S100β, SOX9, GFAP, HEPACAM. |
Basic membrane properties Co-culture with neurons and endothelial cells |
102 |