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. 2021 Nov 2;11(20):10114–10124. doi: 10.7150/thno.62304

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Absence of EV-induced cardiomyocyte proliferation in murine adult hearts. Mean number of proliferating cardiomyocytes (EdU+ and cTnT+) after 7 (A) or 10 (B) days of EdU delivery. Each group consisted of 5-6 randomly selected mice. C. Representative fluorescence microscopic imaging of triple immune-staining for cTnT, GFP and EdU in MerCreMer/ZEG mice. Arrow points to a proliferating cardiomyocyte. D. Cardiomyocyte proliferation in MADM mice expressed as the percentage of single-coloured cardiomyocytes relative to the total number of cardiomyocytes per high-power field and showing similar results in the PBS-injected and EV-CPC-treated groups (n = 5-6). E. Representative fluorescence of bi-photon imaging.