FIGURE 3.

Tet1 deficiency in astrocyte impairs the morphology of neurons in vivo and in vitro. (A) Representative immunostaining images of astrocyte marker Glast and neuronal cell marker Tuj1. WT hippocampal neurons were co-cultured with WT and KO astrocyte, respectively. Scale bar, 100 μm. (B–D) Quantification results showed that neurons co-cultured with KO astrocyte had the decreased dendritic length (B) and number of intersections (C). Sholl analysis showed that neurons co-cultured with KO astrocyte also had the reduced intersection number at distance from soma compared to neurons co-cultured with WT astrocyte. 42 neurons co-cultured with WT astrocytes and 36 neurons co-cultured with Tet1 KO astrocytes were analyzed, respectively. Data were presented as mean ± SEM, unpaired t-test; ***P < 0.001. (E) Representative images of Golgi staining with the brain sections of Ctrl and Tet1 cKO mice. shows the morphology of neuron of WT and Tet1cKO mice in CA1 region. Scale bars, 200 μm (in the upper panels), and 10 μm (in the lower panels). (F) Quantification results showed the decreased spine number of neurons in Tet1 cKO mice compared to Ctrl mice. 10 neurons from Ctrl mice and 9 neurons from cKO mice were analyzed, respectively. Data were presented as mean ± SEM, unpaired t-test; ***P < 0.001.