TABLE 2.

Performance assessment of promoter methylation as biomarker for detection of EOC in tissue and serum samples. Table illustrates the Sensitivity, Specificity, AUC, Accuracy, Optimal Cutoff and Methylation Index of singleplex and multiplex MethyLight assays for the detection of control and epithelial ovarian cancer patients. AUC: Area under the curve; PPV: Positive predictive value; NPV: Negative prediction value; MI: Methylation Index; p-value: Mann-Whitney U test.

Marker	Sensitivity	Specificity	AUC	Optimal cutoff	PPV	NPV	Accuracy	p-value	Methylation index (MI)
TISSUE
HOXA9 a	82.35	85.71	0.92	0.77	99.33	66.67	86.6	<0.00001	0.82
HIC1 a	80.00	77.14	0.85	0.68	89.5	61.4	82.5	<0.00001	0.80
DAPK1	69.41	71.43	0.81	0.49	85.51	49.02	75.8	0.00004	0.69
SOX1	78.82	80.00	0.88	0.38	90.54	60.87	80.8	<0.00001	0.78
RASSF1A	71.76	71.43	0.78	0.42	85.92	51.02	73.3	0.00001	0.72
SFRP1	74.12	71.43	0.80	0.66	86.30	53.19	75.0	<0.00001	0.74
SPARC	61.18	68.57	0.73	0.47	82.54	42.11	73.3	0.00302	0.61
HOXA9+HIC1 a	88.24	88.57	0.92	0.49	94.9	75.6	88.3	<0.00001	0.88
HOXA9+SOX1	85.88	88.57	0.92	0.48	94.81	72.09	87.0	<0.00001	0.86
SOX1+HIC1	83.53	88.57	0.89	0.72	94.67	68.89	87.5	<0.00001	0.83
HIC1+SFRP1	78.82	77.14	0.87	0.75	89.33	60.00	84.0	<0.00001	0.79
RASSF1A+HOXA9	76.47	80.00	0.87	0.49	90.28	58.33	87.5	<0.00001	0.76
DAPK1+SOX1	72.94	77.14	0.87	0.45	88.57	54.00	80.0	<0.00001	0.73
SERUM
HOXA9 a	62.22	100	0.81	0.70	100	59.52	77.1	<0.00001	0.62
HIC1 a	71.11	100	0.88	0.71	100	65.75	82.8	<0.00001	0.71
SOX1	53.33	96	0.77	0.62	96.0	53.33	72.8	0.00003	0.53
HOXA9+SOX1	66.67	96	0.85	0.70	96.77	61.54	88.0	<0.00001	0.67
HOXA9+HIC1 a	88.89	100	0.95	0.64	100	83.33	92.8	<0.00001	0.89
SOX1+HIC1	80.00	96	0.93	0.54	97.30	72.73	87.0	<0.00001	0.80

^aDiagnostic significance of DNA methylation markers-HOXA9 and HIC1 in singleplex and multiplex MethyLight assay analyzed in tissue and serum samples has been previously published (Singh et al., 2020).