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ES cell growth curves. Several six-well plates were prepared with ES cells grown in ES medium. The cells were harvested with trypsin at 20- to 24-h intervals up to 96 h and counted by means of a Coulter counter. The data points and error bars (not all of which are visible) indicate the average cell number and range of three observations. The lines are exponential curves fitted to the data for Capn4+/+ (●), Capn4+/− (▿), and Capn4−/− (■) ES cells.
