Figure 3.

Alteration of intravascular monocyte patrolling behaviour is time-dependent a) Number of detected cells by time-lapse MRI at 5 time points after induction of a peripheral inflammation compared to control mice each 24 h after in vivo labelling of monocytes with ION. b) Ratio of cells with long-term short-range patrolling pattern to all cells detected at 5 time points after induction of a peripheral inflammation compared to control mice each 24h after in vivo labelling with ION. c) Number of detected cells by sequential time-lapse MRI prior to (baseline), 0.5 h and 2 h after sham (left panel) or CLP surgery (right panel) of LysM-GFP mice after in vivo labelling cells with rhodamine-ION. Detailed numbers and statistics can be found in supplementary table 1-4. d) IVM via cranial window of LysM-GFP mice confirmed intravascular presence of in vivo rhodamine-ION-labelled cells (white arrowheads indicate non-moving cells; white arrows indicate patrolling cells). As not all displayed cells were in the focus level and green fluorescence could not be depicted equally in this image, patrolling monocytes were framed. Scale bar = 20 µm. e) IVM analysis of patrolling velocity (µm/s) in Lys-GFP mice after sham or CLP surgery as well as in healthy control mice on a per animal (e) or f) per cell basis. g) IVM analysis of rolling velocity (µm/s) in LysM-GFP mice after sham or CLP surgery as well as in healthy control mice on a per animal (g) or h) per cell basis. *p<0.05, **p<0.01, ***p<0.001.