FIGURE 3.

YbaB_Cc colocalizes with nucleoid in vivo. RP44 cells (CB15N cc2570:pTC67 ftsI(Ts) xylX:pXCFPN-5-ybab_Cc) were cultured at 30°C for 1 h and then shifted to the restrictive temperature (37°C) for 4.5 h in PYE medium, followed by DAPI staining and imaging on agarose-padded glass slides. Fluorescence imaging shows YbaB_Cc-CFP colocalizing with DAPI-stained nucleoid in a temperature-sensitive PBP3 mutant Caulobacter crescentus. DNA-free regions devoid of both DAPI and CFP signal are depicted in circles (yellow).