FIGURE 5.
YbaBCc protects DNA from enzyme degradation. Different concentrations of YbaBCc protein were incubated with supercoiled pBAD18 plasmid DNA, followed by treatment with DNase I restriction enzyme. Control reactions were incubated with BSA in place of YbaBCc protein. YbaBCc prevents complete plasmid DNA degradation by the enzyme as opposed to the BSA control reaction, where DNase I is able to degrade the plasmid completely. Thus indicating that YbaBCc protein-bound plasmid DNA was protected by the degradative action of DNase I restriction enzyme and BSA could not protect the plasmid DNA from being degraded as it does not have DNA-binding activity.