TABLE 1.
Strains and plasmids.
| Relevant genotype or description | Sources/References | |
| C. crescentus | ||
| CB15N | Synchronizable derivative of wild-type strain CB15 (NA 1000) | Evinger and Agabian, 1977 |
| CJW2141 | CB15N cc2570:pTC67 ftsI(Ts) | Costa et al., 2008 |
| RP42 | CB15N xylX:pXCFPN-5-ybabCc | This work |
| RP43 | CB15N/pPAL4 | This work |
| RP45 | CB15N cc2570:pTC67 ftsI(Ts) xylX:pXCFPN-5-ybabCc | This work |
| E. coli | ||
| DH5α | Φ80 ΔlacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA hsdR17 (rk-,mk +) phoA supE44 thi-1 gyrA96 relA1 | Laboratory strain collection |
| S17 | RP4-2, Tc:Mu, KM-Tn7 | Simon et al., 1983 |
| BL21 (DE3) pLysS | F– ompT hsdSB (rB–, mB–) gal dcm (DE3) pLysS(CamR) | Laboratory strain collection |
| MG1655 | K-12 F– λ– ilvG– rfb-50 rph-1 | Laboratory strain collection |
| RP40 | MG1655/pPAL1 | This work |
| RP41 | BL21 (DE3) pLysS/pPAL3 | This work |
| RP44 | BL21 (DE3) pLysS/pPAL5 | This work |
| RP46 | MG1655/pBAD18 | This work |
| Plasmids | ||
| pBAD18 | Kanr, DH5α containing empty pBAD18 | Guzman et al., 1995 |
| pXCFPN-5 | Tetr, vector used for generating N-terminal protein fusions encoded at the xylX locus | Thanbichler et al., 2007 |
| pET28b | Protein expression vector, Kanr | Laboratory strain collection |
| pJS14 | High copy number vector, pBR1MCS derivative, Cmr | Laboratory strain collection |
| pPAL1 | pBAD18 carrying ybabCc, Kanr | This work |
| pPAL2 | pXCFPN-5 carrying ybabCcfused to CFP | This work |
| pPAL3 | pET28b carrying ybabCcfused to 6X-His at N-terminus | This work |
| pPAL4 | pJS14 carrying ybabCc | This work |
| pPAL5 | pET28b carrying truncated ybabCcfused to 6X-His at N-terminus | This work |