TABLE 1.

Galacto-oligosaccharides assay settings and GOS quantification related to GOS synthesis assay.

Enzyme	Reaction set-up				Reaction out-come		Composition of reaction solutions selected for GOS purification
	Initial lactose%	T (°C)	Starting pH	Enzyme concentration (Lu/g)	Slurry clarification	Best GOS yield on dry matter (%)	Galactose (%)	Glucose (%)	Lactulose (%)	Allo-lactose (%)	Lactose (%)	GOS (%)
BgaC	50	40	6.4	4	No
		50	6.2	6	No
		55	6.2	6	No
	40	55	6.2	4,8,12,16	Partial slurry clarification at 11 h with 16 Lu/g	13.1	11.6	16.7	1.0	0.6	57.0	13.1
BgaD	50	40	6.4	4	no
		50	6.2	5, 10, 15	Partial slurry clarification at 12 h with 15 Lu/g
		55	6.2	5, 10, 15	Partial slurry clarification at 5 h with 15 Lu/g
	40	55	6.2	4,8,12,16	At 6 h with enzyme concentration of 12 and 16 Lu/g/no clarification for reaction with 4 and 8 Lu/g	14.7
		55	6.2	20, 30	At 5 h in the reaction with 20 Lu and at 4 h for the reaction with 30 Lu/g	22.4	11.28	17.83	1.14	0.84	46.55	22.4
	30	50	6.2	4,8,16,24	After 30 min for all the concentrations tested	15.2
BgaA	50	50	6.5	3	At 7 h	11.3
		55	6.2	4, 8, 11	At 2 h for the reaction with 8 Lu and after 90 min for the reaction with 11 Lu/g	19	19.7	29.1	0.5	11.4	20.4	19.0
BgaE	50	50	6.5	3	At 7 h	33.7
		55	6.2	2,4,6	at 6 h with 2 Lu/g, at 90 min with 4 Lu and at 1 h with 6 Lu/g	40.3	10.3	25.6	0.1	18.1	5.6	40.3