Figure 4.

Increased senescent photoreceptors in APP23 mice

(A) Representative images of p16^ink4α (marker for cell senescence) labeling on the retinal cross sections of APP23 and control WT mice at 3, 9, and 12 months.

(B and C) Immunofluorescence intensity of p16^ink4α was significantly higher in APP23 mice than in WT mice at 9 months.

(D and E) Immunofluorescence intensity of p16^ink4α as well as p16^ink4α-positive area was significantly higher in APP23 mice than in WT mice at 12 months.

(F) Representative images of p21 (another marker for cell senescence) labeling on the retinal cross sections of APP23 and control WT mice at 3, 9, and 12 months.

(G and H) p21-positive area was significantly higher in APP23 mice than in WT mice at 9 months.

(I and J) p21-positive area was significantly higher in APP23 mice than in WT mice at 12 months. Data are represented as mean ± SEM. ns: not significant, ∗: p < 0.05, ∗∗: p < 0.01, Student's t test. Scale bar = 20 μm