Figure 5.

Increased necrotic photoreceptors in APP23 mice

(A and B) Immunofluorescence labeling of retinal sections using RIPK3-specific antibody showed a higher percentage of RIPK3 positive signals in the OS/IS of photoreceptors in APP23 mice than in WT mice at 12 months.

(C and D) Western blot analysis showed higher expression of RIPK3 in retinas of APP23 mice than in WT mice at 12 months.

(E and F) Immunofluorescence co-labeling of rhodopsin and pRIPK3 (E) or pMLKL (F) (markers for cell necroptosis) in the OS/IS of rods in APP23 and WT mice at 12 months.

(G and H) There are higher pRIPK3-positive and pMLKL-positive signals in the OS/IS of rods in APP23 than in WT mice at 12 months. Data are represented as mean ± SEM. ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001, Student's t test. Scale bar = 20 μm