TABLE 1.

In-house-developed PCR assays for detection of C. pneumoniae^a

Author(s)	Year of publication	Reference	Gene target	Product size (bp)	Type
Holland et al.	1990	31	MOMP	144	S+R
Watson et al.	1991	89	60-kDa protein	582	S+R
Campbell et al.	1992	13	Cloned PstI	474	S+H
Gaydos et al.	1992	21	16S rRNA	463	S (+EIA)
Kaltenboeck et al.	1992	40	MOMP	1,120, 930	M+R (N)
Black et al.	1992	5	MOMP	1,070	S+R
Rasmussen et al.	1992	73	MOMP	145	S+R+H
Tong and Sillis	1993	85	MOMP	333, 207	N
Tjhie et al.	1993	84	MOMP	360, 320, 237	S+N+H
Black et al.	1994	6	16S rRNA	397, 858	N
Kubota	1996	44	53-kDa protein	499	S
Ong et al.	1996	66	MOMP	498, 227	N
Khan et al.	1996	41	DnaK	415	RT
Wilson et al.	1996	93	16S rRNA	317, 178	N
Khan and Potter	1996	42	Cloned PstI	474, 204	N+H
Maass et al.	1997	51	Cloned PstI	474, 128	N+H
Messmer et al.	1997	60	16S rRNA	436, 221	M
Nyström-Rosander et al.	1997	64	16S rRNA	463, 269	N
Yoshida et al.	1998	94	MOMP	258	S+R
Jantos et al.	1998	36	16S rRNA	465	S+EIA
Petitjean et al.	1998	69	60-kDa protein	183	S+EIA
Madico et al.	Submitted	56	16S rRNA	195	S or M

^aAbbreviations: S, single-step PCR; N, nested PCR; M, multiplex PCR; RT, reverse transcriptase; R, restriction enzyme digestion; H, hybridization.