Table 1.

Overview of simulations performed

System	A2aR state	No. of copies of protein	Duration (μs) × repeats	Lipid bilayer composition
9-Copy	inactive	9	50 × 10	Extracellular side: POPC (20%), DOPC (20%), POPE (5%), DOPE (5%), Sph (15%), GM3 (10%), CHOL (25%) Intracellular side: POPC (5%), DOPC (5%), POPE (20%), DOPE (20%), POPS (8%), DOPS (7%), PIP2 (10%), CHOL (25%)
	active		50 × 10
	active + mini Gs		50 × 10
16-Copy	inactive	16	50 × 5
	active		50 × 5
	active + mini Gs		50 × 5
9-Copy NoPIP2	inactive	9	25 × 5	Extracellular side: POPC (20%), DOPC (20%), POPE (5%), DOPE (5%), Sph (15%), GM3 (10%), CHOL (25%) Intracellular side: POPC (5%), DOPC (5%), POPE (25%), DOPE (25%), POPS (8%), DOPS (7%), CHOL (25%)
	active		25 × 5
	active + mini Gs		25 × 5

All simulation boxes were 45 × 45 × 25 nm³. Tails: PO: C16:0/18:1 tails. DO: C16:1, C18:1 tails. PIP₂ molecules had PO tails.