Lysis buffer

Reagent	Final concentration	Amount
NaCl (5 M)	150 mM	3 mL
Tris-HCl (1 M, pH 8.0)	20 mM	2 mL
H2O	n/a	95 mL
Total	n/a	100 mL

Sterile filter and store at room temperature (20°C–25°C).