REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-Tubulin β3 (Tuj1) (1:250)	Biolegend	Cat# ab801202; RRID: AB_10063408
Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:500)	Jackson ImmunoResearch Labs	Cat# 715-545-151; RRID:AB_2341099
Alexa Fluor® 594 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:500)	Jackson ImmunoResearch Labs	Cat# 715-585-151; RRID:AB_2340855
Chemicals, peptides, and recombinant proteins
DMEM	Fisher Scientific	Cat# 11995-040
DPBS	Fisher Scientific	Cat# 14190144
NaCl (5 M)	Fisher Scientific	Cat# 24740011
Tris-HCl (1 M, pH 8.0)	Fisher Scientific	Cat# 15568025
Ketamine	Vedco Inc.	Cat# NDC 50989
Xylazine	Akorn Inc.	Cat# NDC 59399
FBS	Fisher Scientific	Cat# MT35010CV
Penicillin-streptomycin	Fisher Scientific	Cat# BW17602E
Polyethylenimine (PEI)	Polysciences	Cat# 23966-1
Benzonase	Sigma	Cat# E1014_25kU
Iodixanol	Fisher Scientific	Cat# NC1059560
Fast Green	Fisher Scientific	Cat# S25692
Mineral oil	Fisher Scientific	Cat# BP26291
N-Methyl-D-aspartic Acid (NMDA)	Millipore Sigma	Cat# 454575
Fluoromount-G Slide Mounting Medium	Fisher Scientific	Cat# OB100-01
Experimental models: Cell lines
AAVpro 293T Cell Line	Takara Bio	Cat# 632273
Experimental models: Organisms/strains
Mouse: C57BL/6J (8-week-old, male or female)	The Jackson Laboratory	Cat# JAX:000664, RRID: IMSR_JAX:000664
Recombinant DNA
pAAV-GFP	Obtained from Dr. Kevin Park	N/A
pAAV-EBFP	Guo et al. (2021)	N/A
pAAV-CaMKIIα T286D	Guo et al. (2021)	N/A
pRep-Cap	Addgene	Cat# 104963
pHelper	Addgene	Cat# 112867
Software and algorithms
GraphPad Prism 9	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ	Schindelin et al. (2012)	https://imagej.net/Fiji
Other
Confocal microscope	Zeiss	LSM 800
Stereomicroscope	Leica	M60
Motorized microscope stand	Promicra	Promicra PRO-MLS
Beam splitter	Leica	Wild 319449
Microscope camera	HAYEAR	HY-3307
Camera adaptor	Leica	Leica 10445319
Observation tube	Leica-Wild	Wild 411576
Observation tube adaptor	Leica	Wild 10411576
Micropipette puller	Sutter Instruments	Model P-1000
Ultracentrifuge	Beckman	Optima L-90K
Rotor	Beckman	VTi 50
Syringe	Hamilton	Cat# 80965
Bulldog clamp	Fine Scientific Tools	Cat# 18051-28
Mouse head holder	KOPF	Model 921-E
Dumont #55 Forceps	Fine Science Tools	Cat# 11255-20
Dumont #5 Fine Forceps	Fine Science Tools	Cat# 11254-20
Dumont #5 Fine Forceps, only for optic nerve crush	Fine Science Tools	Cat# 11254-20
Scissors	Crimson International Eye instruments	Cat# 6004A-105
Heating pad	ConductScience	Cat# RWD-800-00179-00
OptiSeal centrifuge tube	Beckman	Cat# 362183
15 mL Amicon centrifugal filters	Fisher Scientific	Cat# UFC901024
0.5 mL Amicon centrifugal filters	Fisher Scientific	Cat# UFC501024
PE tubing	Warner Instruments	Cat# 64-0752
Glass capillaries	Sutter Instrument	Cat# BF150-75-10
Underpads	Fisher Scientific	Cat# 22-037-950
Slides	Fisher Scientific	Cat# 22-034979
Cover glass	Fisher Scientific	Cat# 12-542A