TABLE 2.

The mean methylation status of the five genomic regions in the KRAS + and KRAS- samples of replication cohort 1 and cohort 2.

	Genomic Regiona	Geneb	McaMc	McoMc	P valued	FDR	OR	95% CIe	Sensf	Specf	AUCf
KRAS +	6:152129591-152129791	ESR1	0.49	0.06	8.00 × 10–27	9.60 × 10–27	11.00	7.53–15.60	0.96	0.98	0.97
	19:58951599-58951728	ZNF132	0.51	0.03	2.10 × 10–29	1.20 × 10–28	29.00	17.6–47.90	0.98	0.99	1.00
	19:44952604-44952808	ZNF229	0.47	0.11	1.70 × 10–24	1.70 × 10–24	10.20	7.19–14.00	0.90	0.93	0.95
	19:56879613-56879735	ZNF542	0.57	0.13	4.00 × 10–29	1.20 × 10–28	14.30	9.60–21.50	0.99	0.95	0.99
	19:53758048-53758164	ZNF677	0.63	0.25	3.40 × 10–27	5.10 × 10–27	10.90	7.64–15.30	0.90	0.95	0.98
	17:75369456-75369630	SEPT-9	0.58	0.08	2.40 × 10–27	4.70 × 10–27	7.85	5.55–11.00	0.91	0.96	0.98
KRAS-	6:152129591-152129791	ESR1	0.31	0.08	2.60 × 10–18	7.90 × 10–18	4.63	3.21–6.39	0.75	0.83	0.85
	19:58951599-58951728	ZNF132	0.28	0.04	1.30 × 10–19	7.70 × 10–19	6.10	4.09–8.73	0.70	0.97	0.87
	19:44952604-44952808	ZNF229	0.29	0.12	6.90 × 10–10	8.20 × 10–10	3.30	2.20–4.60	0.54	0.94	0.75
	19:56879613-56879735	ZNF542	0.33	0.15	1.50 × 10–10	2.30 × 10–10	2.78	1.87–3.84	0.50	0.96	0.76
	19:53758048-53758164	ZNF677	0.46	0.27	1.80 × 10–09	1.80 × 10–09	2.38	1.62–3.25	0.71	0.73	0.74
	17:75369456-75369630	SEPT-9	0.41	0.11	2.30 × 10–17	4.70 × 10–17	2.94	2.11–3.92	0.77	0.80	0.84
Total	6:152129591-152129791	ESR1	0.39	0.07	1.20 × 10–45	3.50 × 10–45	6.06	4.81–7.52	0.78	0.95	0.91
	19:58951599-58951728	ZNF132	0.39	0.04	6.30 × 10–49	3.80 × 10–48	8.67	6.60–11.20	0.83	0.97	0.93
	19:44952604-44952808	ZNF229	0.37	0.11	3.50 × 10–33	3.50 × 10–33	5.04	3.99–6.24	0.70	0.94	0.85
	19:56879613-56879735	ZNF542	0.44	0.14	1.00 × 10–37	1.50 × 10–37	4.42	3.56–5.39	0.79	0.90	0.87
	19:53758048-53758164	ZNF677	0.54	0.26	1.60 × 10–34	1.90 × 10–34	3.85	3.11–4.67	0.73	0.90	0.86
	17:75369456-75369630	SEPT-9	0.49	0.10	5.50 × 10–45	1.10 × 10–45	4.02	3.27–4.02	0.83	0.87	0.91

^aGenomic region represents the genomic coverage of the reads with targeted bisulfite sequencing, and the genomic coordinates shown here is based on the hg19 version of the genome.

^bThe gene name of the genomic region.

^cMcaM represents the mean methylation percentage of the cases in each region, which consisting of several CpG sites, while the McoM represents the mean methylation percentage of the controls in each region.

^dP value is calculated through the wilcoxon rank-sum test following with FDR (false discovery rate) adjustment for multiple corrections.

^eOR and 95% CI were conducted through logistic regression.

^eSens, sensitivity; while Spec, specificity; AUC, area under curve.

^fThe sensitivity, specificity, and the AUC were calculated through a logistic regression prediction model without adjustment for gender, age and smoking status and alcohol status.