Figure 4.

Uptake of T-VEC-treated dying melanoma cells by BDCA-1⁺/BDCA-3⁺ myDC. 938-mel cells were treated with T-VEC at a MOI of 1 for 24h. Then, cells were harvested, counted and labeled with the pHrodo™ Deep Red Mammalian and Bacterial Cell Labeling Kit. After labeling, labeled T-VEC-treated dying 938-mel cells were put in co-culture with purified BDCA-1⁺/BDCA-3⁺ myDC at the indicated ratios for 2, 4 or 6h. After co-culture for the indicated time points, cells were harvested and analyzed by flow cytometry. The different DC populations were gated and the percentage of pHrodo™-positive cells was determined. Each panel shows the uptake by the indicated DC population. Data are represented as mean ± SD from 3 independent experiments.