Figure 2.

S100A4 acted as a direct target of miR-296-5p. (a) Complementary binding between miR-296-5p and the S100A4 3ʹUTR. (b) A dual-luciferase reporter assay verified the interaction between miR-296-5p and S100A4. *P < 0.05 vs. agomir-NC. (c) S100A4 levels in samples pulled down using biotinylated miR-296-5p were detected by qRT-PCR. **P < 0.01 vs. biotin-NC. (d) After the transfection with the miR-296-5p agomir or antagomir, HUVECs were exposed to ox-LDL (40 µg/mL) for 48 h, and then the S100A4 protein level was detected. *P < 0.05 vs. Blank; ^&P < 0.05 vs. ox-LDL+ agomir-NC; ^#P < 0.05 vs. ox-LDL+ antagomir-NC. (A color version of this figure is available in the online journal.)