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. 2021 Oct 15;13(10):11384–11398.

Figure 2.

Figure 2

LncRNA H19 silencing inhibited the LPS-induced WL-38 cell pyroptosis. A: RT-qPCR was conducted to assess the transfection efficiency; ***P<0.001, LPS vs. the control; ###P<0.001, Si-LncRNA H19+LPS vs. Si-NC+LPS. B: MTT assays were performed to evaluate the WI-38 cell proliferation; **P<0.01, LPS vs. control; #P<0.05, Si-LncRNA H19+LPS vs. Si-NC+LPS. C: TUNEL staining was carried out to assess the WI-38 cell pyroptosis (100×); **P<0.01, LPS vs. control; ##P<0.01, Si-LncRNA H19+LPS vs. Si-NC+LPS. D, E: RT-qPCR and Western blot were conducted to determine the GSDMD and GSDMD-N levels in the WI-38 cells, respectively; *P<0.05, **P<0.01, LPS vs. the control; #P<0.05, Si-LncRNA H19+LPS vs. Si-NC+LPS. F: Immunofluorescence was carried to evaluate the GSDMD-N protein fluorescence intensity in the WI-38 cells (100×). G, H: The IL-1β and IL-18 levels in the WI-38 cells; **P<0.01, LPS vs. the control; #P<0.05, ##P<0.01, Si-LncRNA H19+LPS vs. Si-NC+LPS. I, J: The NLRP3, ASC-1, Caspase-1 levels in the WI-38 cells. **P<0.01, LPS vs. the control; ##P<0.01, Si-LncRNA H19+LPS vs. Si-NC+LPS.