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. 2021 Oct 15;13(10):11384–11398.

Figure 3.

Figure 3

LncRNA H19 overexpression promotes LPS-induced WL-38 cell pyroptosis. A: An RT-qPCR analysis was carried out to determine the LncRNA H19 expression in the WI-38 cells; **P<0.01, LncRNA H19+LPS vs. Lv-NC+LPS. B: MTT assays were conducted to evaluate the WI-38 cell proliferation; *P<0.05, LncRNA H19+LPS vs. Lv-NC+LPS. C: TUNEL staining was carried our to assess the WI-38 cell pyroptosis (100×); *P<0.05, LncRNA H19+LPS vs. Lv-NC+LPS. D, E: The GSDMD and GSDMD-N levels in the WI-38 cells; **P<0.01, LncRNA H19+LPS vs. Lv-NC+LPS. F: Immunofluorescence was performed to assess the GSDMD-N protein fluorescence intensity in the WI-38 cells (100×); G, H: The IL-1β and IL-18 levels in the WI-38 cells; *P<0.05, **P<0.01, LncRNA H19+LPS vs. Lv-NC+LPS. I, J: The NLRP3, ASC-1, and Caspase-1 levels. *P<0.05, **P<0.01, LncRNA H19+LPS vs. Lv-NC+LPS.