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. 2021 Oct 15;13(10):11384–11398.

Figure 5.

Figure 5

NLRP3 attenuates the effect of LncRNA H19 on the LPS-induced WL-38 cell pyroptosis. A: RT-qPCR and Western blot were conducted to assess the NLRP3 levels; **P<0.01, ***P<0.001, vs. Si-NC. B: MTT assays were performed to evaluate the WI-38 cell proliferation; *P<0.05, miR-22-3p inhibitor+NC vs. NC inhibitor+si-NC; #P<0.05, miR-22-3p+si-NLPR3 vs. miR-22-3p inhibitor+si-NC. C: TUNEL staining was carried to measure the WI-38 cell pyroptosis (100×); **P<0.01, miR-22-3p inhibitor+NC vs. NC inhibitor+si-NC; ##P<0.01, miR-22-3p+si-NLPR3 vs. miR-22-3p inhibitor+si-NC. D, E: RT-qPCR and Western blot were conducted to measure the levels of the pyroptosis-related genes, including GSDMD, GSDMD-N, NLRP3, ASC-1 and Caspase-1; **P<0.01, miR-22-3p inhibitor+NC vs. NC inhibitor+si-NC; #P<0.05, ##P<0.01, miR-22-3p+si-NLPR3 vs. miR-22-3p inhibitor+si-NC. F, G: RT-qPCR and ELISA analyses were performed to assess the IL-1β and IL-18 levels. **P<0.01, miR-22-3p inhibitor+NC vs. NC inhibitor+si-NC; #P<0.05, miR-22-3p+si-NLPR3 vs. miR-22-3p inhibitor+si-NC.