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. 2021 Oct 15;13(10):11450–11460.

Figure 2.

Figure 2

The overexpression of miR-21-5p inhibits inflammatory injuries in LPS-treated H9c2 cells. miR-NC and miR-21-5p were transfected in the LPS-treated H9c2 cells. A. A qRT-PCR analysis of the expression of miR-21-5p. B-D. The cytokines were measured using ELISA. E. The cell viability was measured using CCK-8 assays. F. EdU assays were used to analyze the cell proliferation (400×). G. TUNEL assays were used to analyze the apoptosis (400×). H. Western blot was used to analyze the expression levels of the apoptosis associated proteins. The values are the mean ± sd, *P<0.05, **P<0.01, ***P<0.001 vs. LPS + miR-NC, n=3 per group.