Figure 2.

Cytotoxic effect of LDN193189 against mouse melanoma cells (B16F10). For MTT assay, B16F10 cells were treated with LDN193189 (0.25-5 μM) for 72 h. Data shown are mean ± SD of three replicate wells. A graph was plotted with concentration of LDN193189 (X-axis) vs. cell viability % (Y-axis) and the IC₅₀ values were calculated using the formula y=b+ax. An IC₅₀ value is the concentration of the drug which results in 50% cell death.