Table 1.

Genes and the fold change of proteins altered in the LDN193189 treated melanoma tissue

Sl no	Up-regulated (fold change ≥1.5)			Down-regulated (fold change ≤0.66)
	Gene Symbol	Description	Fold change	Gene Symbol	Description	Fold change
1	Car2	carbonic anhydrase 2 isoform X1	7.813	Mt2	metallothionein-2	0.266
2	Hbb-bt	hemoglobin, beta adult t chain	6.061	Ccdc126	coiled-coil domain-containing protein 126 isoform 1 precursor	0.276
3	Pvalb	parvalbumin alpha isoform X1	4.565	Mt1	metallothionein-1	0.286
4	Hba-a2	hemoglobin alpha, adult chain 2	4.242	Yaf2	YY1-associated factor 2 isoform X1	0.334
5	Scrn3	secernin-3 isoform X1	3.952	Tfeb	transcription factor EB isoform a	0.355
6	Slc4a1	band 3 anion transport protein	3.933	Serpina1e	alpha-1-antitrypsin 1-5 precursor	0.361
7	Fga	fibrinogen alpha chain isoform 1 preproprotein	3.582	Hist2h2aa2	histone H2A type 2-A	0.477
8	Fgg	fibrinogen gamma chain isoform 2 precursor	3.427	Hacd2	3-Hydroxyacyl-CoA Dehydratase 2	0.477
9	Fgb	fibrinogen beta chain preproprotein	3.360	Hspb1	heat shock protein beta-1	0.485
10	Tnnc2	troponin C, skeletal muscle	3.155	Hmga1	high mobility group protein HMG-I/HMG-Y isoform a	0.527

The data shown in the table are the altered proteins in the LDN193189 treated as compared to the untreated controls. The data obtained for LC-MS/MS were analyzed using Proteome discoverer 2.1, which compares the LC-MS/MS data against mouse database. This table shows 10 proteins with highest fold increase and 10 proteins which are most down-regulated, their gene symbol, and their fold change, i.e., concentration in LDN193189 treated melanoma/concentration in untreated melanoma.