Table 3. Detection of TTX in Pufferfish Extracts.
TTXs content
(L. sceleratus)b(mg TTX equiv/kg) |
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hybrid antibody–aptamer sandwich assay |
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tissue | % TTX recovery (L. lagocephalus)a | PBS | extract | competitive magnetic bead ELISA |
gonads | 109.1 | 9.46 | 9.94 | 5.24 |
liver | 93.5 | 5.99 | 5.01 | 2.84 |
skin | 107.7 | 0.98 | 1.28 | 0.19 |
muscle | 96.3 | 0.86 | 0.82 | 0.42 |
Recovery (%) of TTX spiked in diluted extracts from a TTX-free fish (L. lagocephalus).
TTX content (mg TTX equiv/kg of tissue) in extracts from a TTX-containing fish (L. sceleratus) was determined using calibration curves constructed in PBS buffer and in the respective extract from the TTX-free fish.