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. 2021 Oct 26;93(44):14810–14819. doi: 10.1021/acs.analchem.1c03671

Table 3. Detection of TTX in Pufferfish Extracts.

    TTXs content (L. sceleratus)b(mg TTX equiv/kg)
hybrid antibody–aptamer sandwich assay
 
tissue % TTX recovery (L. lagocephalus)a PBS extract competitive magnetic bead ELISA
gonads 109.1 9.46 9.94 5.24
liver 93.5 5.99 5.01 2.84
skin 107.7 0.98 1.28 0.19
muscle 96.3 0.86 0.82 0.42
a

Recovery (%) of TTX spiked in diluted extracts from a TTX-free fish (L. lagocephalus).

b

TTX content (mg TTX equiv/kg of tissue) in extracts from a TTX-containing fish (L. sceleratus) was determined using calibration curves constructed in PBS buffer and in the respective extract from the TTX-free fish.