TABLE 2.
Oligonucleotide primers used for the PCR-LIF and PCR-REA systems
Name | Position in the 16S rRNA genesa | Sequence |
---|---|---|
F-SCAP | 1225–1249 | 5′-Cy5-GTACAAAGAGTTGCAATCCTGTGAA-3′ |
R-SCAP | 1353–1330 | 5′-ACCGCGACATAGCTGATTCGCGA-3′ |
F-REAP | 143–168 | 5′-GAAACGAAAGATAATACCGCATGTAG-3′ |
R-REAP | 927–907 | 5′-CCACTTGTGCGGGTCCCCGTC-3′ |
Numbering conforms to the consensus sequence of the 16S rRNA genes of the M. mycoides cluster (22).