TABLE 3.
Results for specimens which were applied to chromatography paper and analyzed by PCR-LIF and PCR-REA
| Specimen no. (herd) | Specimen type | Country | Result of:
|
Postmortem observations | Commentsa | Mycoplasma growth in culture | GI testb result | |
|---|---|---|---|---|---|---|---|---|
| PCR-LIF | PCR-REA | |||||||
| 1 (a) | Pleural fluid | Tanzania | + | + | Typical CBPP lesions | |||
| 2 (a) | Pleural fluid | Tanzania | + | + | Typical CBPP lesions | |||
| 3 (b) | Lung | Kenya | − | − | No CBPP lesions | |||
| 4 (c) | Lung | Kenya | + | + | Typical CBPP lesions | Treated with antibiotics | + | + |
| 5 (c) | Lung effusion | Kenya | + | +c | Typical CBPP lesions | From the same animal as specimen 4 | + | + |
| 6 (c) | Lung | Kenya | − | − | No CBPP lesions | Positive by CFT and cELISA | − | |
| 7 (c) | Lung | Kenya | − | − | Normal appearance | Positive by CFT and cELISA | − | |
| 8 (c) | Lung | Kenya | + | +c | Typical CBPP lesions | Positive by CFT and cELISA | − | |
| 9 (d) | Lung | Kenya | − | − | Positive by CFT and cELISA | − | ||
| 10 (c) | Lung | Kenya | + | + | Typical CBPP lesions | + | + | |
| 11 (e) | Lung | Uganda | − | − | Pneumonia | + | − | |
| 12 (e) | Lung | Uganda | − | − | Pneumonia | − | ||
| 13 (e) | Lung | Uganda | − | − | Pneumonia | − | ||
| 14 (e) | Lung | Uganda | − | − | Pneumonia | − | ||
| 15 (c) | Culture of specimen 10 | + | + | + | + | |||
| 16 (a) | Lung tissued | Tanzania | + | + | Typical CBPP lesions | − | ||
a
CFT, complement fixation test; cELISA, competitive ELISA.
b
GI test, growth inhibition test for M. mycoides subsp. mycoides.
c
Nested PCR was required to produce an amount of amplicon that was sufficient for identification of M. mycoides SC after AluI restriction.
d
The sample was not applied to chromatography paper; DNA was extracted from frozen lung tissue.