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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Immunol. 2020 Dec 7;206(1):101–108. doi: 10.4049/jimmunol.2000576

Figure 2: Older males display a lower level of A/T mutability in SHM.

Figure 2:

A/T mutability was calculated by normalizing frequency of unselected mutations occurring on A or T nucleotides by frequency of A or T bases in germline immunoglobulin sequences for each individual in the AIRR-seq dataset. Unselected mutations were obtained from silent mutations in functional sequences (Functional) or all mutations in non-functional sequences (Non-functional). Boxes represent the median and interquartile range of A/T mutabilities in each age and sex group. The analysis was performed in all three technical replicates: RNA (left panels), DNA-GC (middle panels), DNA-Roche (right panels). p-values were obtained from Welch’s t-test.