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Immunogold labelling of intimin. The specificity of the Int388–667 antibody was confirmed by labelling wild-type EPEC strain E2348/69 (a) and its intimin-negative derivative, CVD206 (b). The antiserum labelled all strains that express intimins α, β, and δ, although there was some strain-to-strain variation in the level of intimin expression illustrated here, with two strains expressing intimins α (c) and β (d), respectively. Magnification, ×30,000.
