FIG. 3.

Detection of different intimin types with the universal intimin primers. A specific PCR product (840 bp) was generated from a human O127 EPEC isolate expressing intimin α (lane 2), cow O26 isolates expressing intimin β (lanes 4 and 8), sheep O157:H7 and O157:H− isolates expressing intimin γ (lanes 5 and 6, respectively), a cow O157:H7 isolate expressing intimin γ (lane 7), and a pig O26 isolate expressing intimin β (lane 10). No PCR product was obtained with CVD206 (lane 3), a cow O26 isolate expressing intimin β (lane 9), L. monocytogenes (lane 11), or Y. pseudotuberculosis (lane 12). Molecular size markers (1-kb ladder) were loaded in lane 1.