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. 2021 Nov 11;21:1201. doi: 10.1186/s12885-021-08930-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — DNA damage assessed by histone γH2AX and 53BP1 foci counts per nucleus in control or drug-treated and/or irradiated M059K (a) and M059J (b) cells. Cells were treated with PI-103 3 h prior to irradiation with 2 Gy, fixed at different time points (30 min, 2 h, 4 h and 6 h) after irradiation and immunostained for γH2AX and 53BP1. DNA damage was assessed by the mean number of γH2AX (green) and 53BP1 (magenta) foci per nucleus in non-irradiated and irradiated (2 Gy) cells, either in control or in cells treated with PI-103 fixed 30 min, 2 h, 4 h and 6 h post-irradiation. For each time point at least 100 nuclei were evaluated using the ImageJ plugin FocAn [28]