FIG. 7.

Expression of Clb2p and Clb5p from the GAL1 promoter results in comparable levels of protein, associated Cdc28p, and in vitro kinase activity. All strains have the indicated construct integrated in 1255-5C (wild type [WT]). Strains were grown overnight in YEPRaf. After 4 h of induction of the GAL1 promoter with 3% galactose at 30°C, the cultures were processed for immunoprecipitation of the HA-tagged proteins, followed by an in vitro kinase assay of the immunoprecipitates. Protein samples were analyzed by Western blotting with antibodies against either the HA tag or Cdc28p. ³²P-phosphorylated histone-H1 (H1-³²P) was detected by autoradiography.