TABLE 1.

Budding and rebudding percentages resulting from GAL1-induced CLB2 and CLB5 constructs

Genotype	% of cellsa
	Expt 1		Expt 2
	Budded	Rebudded	Budded	Rebudded
Set I
Wild type	67	0	68	0
GAL1::CLB2	59	0	60	0
GAL1::CLB2-Δdb	88	0	90	0
GAL1::CLB5	67	2	68	2
GAL1::CLB5-Δdb	56	39	56	42
Set II
Wild type + vector	54	2	55	5
GAL1::CLB5-Δdb + vector	68	27	69	28
GAL1::CLB5-Δdb + pGAL1::CLB2	64	22	48	24
GAL1::CLB5-Δdb + pGAL1::CLB2-Δdb	54	4	53	12

^aPercentages of budded and rebudded cells were determined by counting 200 cells for each sample in two separate experiments. The samples were processed by the methods described for Fig. 7 (set I) and 8 (set II) following 6 h of galactose induction. Budded cells represent only singly budded cells, and rebudded cells represent all variations of multiply budded cells (typically either three or four cell bodies).