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. 2021 Nov 11;164:13–16. doi: 10.1016/j.yjmcc.2021.11.003

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Fig. 1 — Assessment of ACE2 and TMPRSS2 across sex and aging.

A. ACE2 mRNA expression (n = 36, biological replicates), protein levels (n = 11, biological replicates), and activity (n = 36, biological replicates) are positively correlated across all organs in male and female animals (B.). C. TMPRSS2 mRNA (n = 36, biological replicates) and protein levels (n = 11, biological replicates). Data from A-C was generated from C57BL6/J mice using a pooled analysis of males and females. Levels are compiled from all sexes and age groups for expression and activity; however, protein levels are compiled from representative and three replicate gels sampled from a subset of all age groups (Supplementary Fig. S1). All organs were run on the same gel for comparison. Immunoblots were over-exposed (O.E.) to visualize low levels in the lung and heart. Expression and activity data were run one time in the same plate for quantification. Data are represented as median with interquartile range (IQR) and were analyzed as independent samples with the Kruskal-Wallis test with pairwise comparison adjusted by Bonferroni correction; * indicates differences from the heart; # indicates differences from the lung; and † indicates differences from the kidney. *p < 0.05; **p < 0.01, ***p < 0.001 where the number of symbols indicates the level of significance. Analysis of mRNA expression (D.), protein levels (E.), and ACE2 activity (F.) of ACE2 and TMPRSS2 across sex and age in the heart (magenta), lung (green), kidney (yellow), and small intestine (SI) (blue) (n = 6, biological replicates/group). ACE2 global knockout mice were analyzed as a negative control (KO) for western blots. Raw images are provided in Supplementary Fig. S2-S3. Data are represented as the mean ± SEM and analyzed by two-way ANOVA with Tukey post hoc test (mRNA and activity), or unpaired student's t-test (protein levels). For all experiments, young animals (n = 12) were obtained from four litters and each litter was collected over two days. Adult and aged animals (n = 12 /group) were obtained from five and three litters respectively, and each litter was collected over two days. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)