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. 2021 Oct 28;12:705086. doi: 10.3389/fimmu.2021.705086

Figure 5.

Figure 5

PMEPA1 promotes bladder cancer cell growth, colony formation (CF) abilities, and cell migration and invasion in vitro. (A) The efficiency of PMEPA1 knocked down and overexpressed has been testified by Western blot in T24 and 647-V. (B) The proliferation of T24 and 647-V cells with PMEPA1 knocked down and overexpressed was detected by CCK8 assay (n = 3). (C) The proliferation of T24 and 647-V cells with PMEPA1 knocked down and overexpressed was detected by plate clone assay (n = 3). (D) The cell migration of T24 and 647-V cells with PMEPA1 knocked down and overexpressed was detected by wound healing assays (n = 3). (E) The cell migration and invasion of T24 and 647-V cells with PMEPA1 knocked down and overexpressed was detected by transwell assays (n = 3). The * markers indicate the statistical analysis is between PMEPA1 knocked down, overexpressed and vector. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.