Figure 4.

(a) Fluorescent images of harvested tumors from mice that were sacrificed at 3 h after co-injection of untargeted 650z probe and one of the three targeted s775z probes. Fluorescence filter settings for the untargeted dye (ex: 640/20 nm, em: 710/20 nm, exposure time: 3 s, percent power: 50%, F-stop: 2, FOV: 20, binning—low) and targeted probe (ex: 745/20 nm, em: 850/20 nm, exposure time: 3 s, percent power: 50%, F-stop: 2, FOV: 20, binning—low) and the fluorescence intensity scale is in arbitrary units. (b) (left) Plots of tumor and muscle BP for each s775z probe in the excised tumor and muscle tissue harvested from each mouse (N = 5). (right) Pixel intensity maps showing the heterogenous distribution of BP for each s775z probe in the excised tumors.