Table 1. Spectral Properties of Fluorescent Probes in pH 7.4 PBS Buffer at Room Temperaturea.
Dye | λmaxabs (nm) | λmaxem (nm) | SS (nm) | ε (M–1 cm–1) | R2 | ΦF (%)b | brightnessb |
---|---|---|---|---|---|---|---|
s775z | 775 | 794 | 19 | 201,000 | 0.999 | 9.0 | 18,000 |
s775z-aRGD | 776 | 796 | 20 | 190,000 | 0.999 | 8.1 | 15,000 |
s775z-sRGD | 776 | 796 | 20 | 200,000 | 0.996 | 8.2 | 16,000 |
s775z-tRGD | 776 | 797 | 21 | 170,000 | 0.995 | 8.1 | 14,000 |
650z | 640 | 659 | 19 | 270,500 | 22.5 | 61,000 |
SS is the Stokes shift, ε is molar absorptivity, ΦF is fluorescence quantum yield, brightness = ε × ΦF.
Estimated error is ±10%.