Figure 4.
Representative image of a malachite green assay of TTn analogue 1 d run at various concentrations on a 96-well plate with (+)-control (no inhibitor) and (−)-control (no inhibitor or K–R–pT–I–R–R). Phophorylase activity of PP1 and PP2A can be visualized through green (active) or yellow (inhibited) well coloration due to the presence or absences of malachite green-molybdate-phosphate complex.