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. Author manuscript; available in PMC: 2021 Nov 11.
Published in final edited form as: Dev Dyn. 2019 Sep 10;248(12):1195–1210. doi: 10.1002/dvdy.106

Figure 6. Ztmem2 mutants exhibit excess HA deposition.

Figure 6.

Immunofluorescence with MF20 (red) to label the myocardium and HABP (green) to label HA in single optical slices through wt (A-C) and Ztmem2 mutant (D-F) hearts at 48 hpf. In wt, a thin layer of HA is visible throughout the ventricle, adjacent to the myocardium, and increased concentration of HA is visible within the AVC (A, arrowheads). In contrast, Ztmem2 mutants display increased levels of HA throughout the ventricle and at the AVC (D, arrowheads). Separate optical slices (G-L), 2.5 microns away from those shown in (A-F), provide a better view of the inferior portion of the AVC in the same wt (G-I) and Ztmem2 mutant (J-L) hearts. V and A indicate the ventricle and atrium, respectively. For wt, n=8; for Ztmem2, n=6.